A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.
/mnt/fastcomputedata/ariadna/prod/nextflow/work/2237/e3/902bee4b2fcac407adb8eaaf2a67dc
General Statistics
| Sample Name | Total reads | Aligned | Aligned | Uniq aligned | Uniq aligned | Multimapped |
|---|---|---|---|---|---|---|
| AGDS_DS384_Huh7_TGFb_SB_MOA_01 | 475.7M | 331.7M | 69.7% | 331.7M | 69.7% | 0.0M |
| AGDS_DS384_Huh7_TGFb_SB_MOA_02 | 354.5M | 287.1M | 81.0% | 287.1M | 81.0% | 0.0M |
STAR
Universal RNA-seq aligner.https://github.com/alexdobin/STARDOI: 10.1093/bioinformatics/bts635
Summary Statistics
Summary statistics from the STAR alignment
| Sample Name | Total reads | Aligned | Aligned | Uniq aligned | Uniq aligned | Multimapped | Avg. read len | Avg. mapped len | Splices | Annotated splices | GT/AG splices | GC/AG splices | AT/AC splices | Non-canonical splices | Mismatch rate | Del rate | Del len | Ins rate | Ins len |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGDS_DS384_Huh7_TGFb_SB_MOA_01 | 475.7M | 331.7M | 69.7% | 331.7M | 69.7% | 0.0M | 98.0bp | 97.7bp | 74.5M | 73.4M | 73.7M | 0.3M | 0.0M | 0.5M | 0.6% | 0.0% | 1.6bp | 0.0% | 1.3bp |
| AGDS_DS384_Huh7_TGFb_SB_MOA_02 | 354.5M | 287.1M | 81.0% | 287.1M | 81.0% | 0.0M | 98.0bp | 98.1bp | 57.4M | 56.4M | 56.7M | 0.2M | 0.0M | 0.4M | 0.6% | 0.0% | 1.6bp | 0.0% | 1.3bp |
Alignment Scores
Gene Counts
Statistics from results generated using --quantMode GeneCounts. The three tabs show counts for unstranded RNA-seq, counts for the 1st read strand aligned with RNA and counts for the 2nd read strand aligned with RNA.