Library preparation and sequencing data analysis report
Project name (ID): AMP0001
Overview
Library preparation summary
Library name
Number of samples
SP_L060526DEMO1_01
15
SP_L060526DEMO2_01
15
SP_L060526DEMO3_01
15
SP_L060526DEMO4_01
15
Library sequencing summary
PASSWARNING
Library? FASTQ name of the BRB-seq library
PF_reads? The total number of reads
Avg. nb. reads/sample? Defined as the number of demultiplexed reads / used samples
q30 R1? Rate of bases in Read 1 (BC+UMI) with Q >= 30
q30 R2? Rate of bases in Read 2 (genomic) with Q >= 30
SP_L060526DEMO1_01
447348842
28,680,783
0.95611
0.953188
SP_L060526DEMO2_01
509821122
32,634,096
0.954463
0.951647
SP_L060526DEMO3_01
470545249
30,269,967
0.953808
0.950979
SP_L060526DEMO4_01
452824856
29,118,814
0.955008
0.952618
Library alignment summary
PASSWARNING
Library? FASTQ name of the BRB-seq library
Genome assembly? Reference genome used for alignment
Nb. Mapped? Total number of reads mapped against the genome
% Mapped? % of the reads mapped against the genome
Nb. Mapped to exons? Total number of quantified reads
% Exons? Total % of quantified counts
Nb. genes? Average number of detected genes across used samples
Nb. ERCC? Total number of reads mapped to ERCC spike-ins
% ERCC? Total % of reads mapped to ERCC spike-ins
SP_L060526DEMO1_01
homo_sapiens GRCh38 114
357,768,310
83.16
318,913,387
74.13
19898.40
1,849
0
SP_L060526DEMO2_01
homo_sapiens GRCh38 114
411,004,072
83.96
366,455,751
74.86
20786.67
2,082
0
SP_L060526DEMO3_01
homo_sapiens GRCh38 114
381,857,768
84.1
340,765,589
75.05
20426.13
1,866
0
SP_L060526DEMO4_01
homo_sapiens GRCh38 114
366,195,522
83.84
326,592,913
74.77
20714.07
1,808
0
SP_L060526DEMO1_01
Number of sequencing reads, per sample - SP_L060526DEMO1_01? Per sample identification statistics, presented in barplot and plate view. The order of samples in barplot can be changed to default, row-wise and column-wise.
Hover for detailed sample information.
Alignment statistics, per sample - SP_L060526DEMO1_01? Per sample total alignment statistics perfomed by STARsolo, presented in barplot and plate view. The order of samples in barplot can be changed to default, row-wise and column-wise.
Hover for detailed sample information.
Number of detected genes, per sample - SP_L060526DEMO1_01? Per sample gene detection statistics, presented in barplot and plate view. The order of samples in barplot can be changed to default, row-wise and column-wise.
Hover for detailed sample information.
Compare sample-wise statistics - SP_L060526DEMO1_01? This scatter plot enables comparison of various individual statistics, such as the number of reads, genes, alignment percentage, and ERCC counts per sample. Both the x and y axes can be customized using drop-down menus to explore relationships between these metrics.
Hover for detailed sample information.
Principle Component Analysis - SP_L060526DEMO1_01? The PCA plot displays the general expression profiles of all samples, with dots sized according to read counts. It helps identify patterns and clusters in gene expression, showing how samples with similar or differing profiles group together based on their overall expression patterns.
This plot was generated using truncated PCA (irlba).
Hover for detailed sample information.
Top-10 most expressed genes across samples - SP_L060526DEMO1_01? The pie chart shows the top 10 most highly expressed genes, with each slice representing their percentage of total gene expression.
Hover for detailed sample information.
Top-10 most expressed biotypes across samples - SP_L060526DEMO1_01? The pie chart shows the top 10 most highly expressed gene biotypes, with each slice representing their percentage of total gene expression.
Hover for detailed sample information.
SP_L060526DEMO2_01
Number of sequencing reads, per sample - SP_L060526DEMO2_01? Per sample identification statistics, presented in barplot and plate view. The order of samples in barplot can be changed to default, row-wise and column-wise.
Hover for detailed sample information.
Alignment statistics, per sample - SP_L060526DEMO2_01? Per sample total alignment statistics perfomed by STARsolo, presented in barplot and plate view. The order of samples in barplot can be changed to default, row-wise and column-wise.
Hover for detailed sample information.
Number of detected genes, per sample - SP_L060526DEMO2_01? Per sample gene detection statistics, presented in barplot and plate view. The order of samples in barplot can be changed to default, row-wise and column-wise.
Hover for detailed sample information.
Compare sample-wise statistics - SP_L060526DEMO2_01? This scatter plot enables comparison of various individual statistics, such as the number of reads, genes, alignment percentage, and ERCC counts per sample. Both the x and y axes can be customized using drop-down menus to explore relationships between these metrics.
Hover for detailed sample information.
Principle Component Analysis - SP_L060526DEMO2_01? The PCA plot displays the general expression profiles of all samples, with dots sized according to read counts. It helps identify patterns and clusters in gene expression, showing how samples with similar or differing profiles group together based on their overall expression patterns.
This plot was generated using truncated PCA (irlba).
Hover for detailed sample information.
Top-10 most expressed genes across samples - SP_L060526DEMO2_01? The pie chart shows the top 10 most highly expressed genes, with each slice representing their percentage of total gene expression.
Hover for detailed sample information.
Top-10 most expressed biotypes across samples - SP_L060526DEMO2_01? The pie chart shows the top 10 most highly expressed gene biotypes, with each slice representing their percentage of total gene expression.
Hover for detailed sample information.
SP_L060526DEMO3_01
Number of sequencing reads, per sample - SP_L060526DEMO3_01? Per sample identification statistics, presented in barplot and plate view. The order of samples in barplot can be changed to default, row-wise and column-wise.
Hover for detailed sample information.
Alignment statistics, per sample - SP_L060526DEMO3_01? Per sample total alignment statistics perfomed by STARsolo, presented in barplot and plate view. The order of samples in barplot can be changed to default, row-wise and column-wise.
Hover for detailed sample information.
Number of detected genes, per sample - SP_L060526DEMO3_01? Per sample gene detection statistics, presented in barplot and plate view. The order of samples in barplot can be changed to default, row-wise and column-wise.
Hover for detailed sample information.
Compare sample-wise statistics - SP_L060526DEMO3_01? This scatter plot enables comparison of various individual statistics, such as the number of reads, genes, alignment percentage, and ERCC counts per sample. Both the x and y axes can be customized using drop-down menus to explore relationships between these metrics.
Hover for detailed sample information.
Principle Component Analysis - SP_L060526DEMO3_01? The PCA plot displays the general expression profiles of all samples, with dots sized according to read counts. It helps identify patterns and clusters in gene expression, showing how samples with similar or differing profiles group together based on their overall expression patterns.
This plot was generated using truncated PCA (irlba).
Hover for detailed sample information.
Top-10 most expressed genes across samples - SP_L060526DEMO3_01? The pie chart shows the top 10 most highly expressed genes, with each slice representing their percentage of total gene expression.
Hover for detailed sample information.
Top-10 most expressed biotypes across samples - SP_L060526DEMO3_01? The pie chart shows the top 10 most highly expressed gene biotypes, with each slice representing their percentage of total gene expression.
Hover for detailed sample information.
SP_L060526DEMO4_01
Number of sequencing reads, per sample - SP_L060526DEMO4_01? Per sample identification statistics, presented in barplot and plate view. The order of samples in barplot can be changed to default, row-wise and column-wise.
Hover for detailed sample information.
Alignment statistics, per sample - SP_L060526DEMO4_01? Per sample total alignment statistics perfomed by STARsolo, presented in barplot and plate view. The order of samples in barplot can be changed to default, row-wise and column-wise.
Hover for detailed sample information.
Number of detected genes, per sample - SP_L060526DEMO4_01? Per sample gene detection statistics, presented in barplot and plate view. The order of samples in barplot can be changed to default, row-wise and column-wise.
Hover for detailed sample information.
Compare sample-wise statistics - SP_L060526DEMO4_01? This scatter plot enables comparison of various individual statistics, such as the number of reads, genes, alignment percentage, and ERCC counts per sample. Both the x and y axes can be customized using drop-down menus to explore relationships between these metrics.
Hover for detailed sample information.
Principle Component Analysis - SP_L060526DEMO4_01? The PCA plot displays the general expression profiles of all samples, with dots sized according to read counts. It helps identify patterns and clusters in gene expression, showing how samples with similar or differing profiles group together based on their overall expression patterns.
This plot was generated using truncated PCA (irlba).
Hover for detailed sample information.
Top-10 most expressed genes across samples - SP_L060526DEMO4_01? The pie chart shows the top 10 most highly expressed genes, with each slice representing their percentage of total gene expression.
Hover for detailed sample information.
Top-10 most expressed biotypes across samples - SP_L060526DEMO4_01? The pie chart shows the top 10 most highly expressed gene biotypes, with each slice representing their percentage of total gene expression.
